The second generation ultrasound contrast agents (UCA/USCA) are both sufficiently small and stable to pass into the systemic circulation, and these contrast media enhance the Doppler signal in various arteries after intravenous injection. Second generation agents have a short live, the contrast effect is over in a few minutes.

The earliest introduction of vascular ultrasound contrast agents (USCA) was by Gramiak and Shah in 1968, when they injected agitated saline into the ascending aorta and cardiac chambers during echocardiographic to opacify the left heart chamber. Strong echoes were produced within the heart, due to the acoustic mismatch between free air microbubbles in the saline and the surrounding blood.
The disadvantage of this microbubbles produced by agitation, was that the air quickly leak from the thin bubble shell into the blood, where it dissolved. In addition, the small bubbles that were capable of traversing the capillary bed did not survive long enough for imaging because the air quickly dissipated into the blood. Aside from agitated saline, also hydrogen peroxide, indocyanine green dye, and iodinated contrast has been tested. The commercial development of contrast agents began in the 1980s with greatest effort to the stabilization of small microbubbles.

The development generations by now:
To pass through the lung capillaries and enter into the systemic circulation, microspheres should be less than 10 μm in diameter. Air bubbles in that size range persist in solution for only a short time; too short for systemic vascular use.
The first developed agent was Echovist (1982), which enabled the enhancement of the right heart. The second generation of echogenic agents, sonicated 5% human albumin-containing air bubbles (Albunex), were capable of transpulmonary passage but often failed to produce adequate imaging of the left heart. Both Albunex and Levovist utilize air as the gas component of the microbubble.
In the 1990s newer developed agents with fluorocarbon gases and albumin, surfactant, lipid, or polymer shells have an increased persistence of the microspheres. This smaller, more stable microbubble agents, and improvements in ultrasound technology, have resulted in a wider range of application including myocardial perfusion.

See also First Generation USCA, Second Generation USCA, and Third Generation USCA.

Sound waves must have a medium to pass through. The velocity or propagation speed is the speed at which sound waves travel through a particular medium measured in meters per second (m/s) or millimeters per microsecond (mm/μs). Because the velocity of ultrasound waves is constant, the time taken for the wave to return to the probe can be used to determine the depth of the object causing the reflection.
The velocity is equal to the frequency x wavelength.
V = f x l
The velocity of ultrasound will differ with different media. In general, the propagation speed of sound through gases is low, liquids higher and solids highest. The speed of sound depends strongly on temperature as well as the medium through which sound waves are propagating. At 0 °C (32 °F) the speed of sound in air is about 331 m/s (1,086 ft/s; 1,192 km/h; 740 mph; 643 kn), at 20 °C (68 °F) about 343 metres per second (1,125 ft/s; 1,235 km/h; 767 mph; 667 kn)

Velocity (m/s)

Doppler ultrasound visualizes blood flow-velocity information. The peak systolic velocity and the end diastolic velocity are major Doppler parameters, which are determined from the spectrum obtained at the point of maximal vessel narrowing. Peak systolic velocity ratios are calculated by dividing the peak-systolic velocity measured at the site of flow disturbance by that measured proximal of the narrowing (stenosis, graft, etc.).

See Acceleration Index, Acceleration Time, Modal Velocity, Run-time Artifact and Maximum Velocity.

(N) The SI unit of force.
Definition: 1 Newton will accelerate a mass of 1 kilogram at the rate of 1 meter per second per second.
The relationship between force (F), mass (m), and acceleration (a) is expressed by the formula F = ma.
The Newton is named for Isaac Newton (1642-1727), the British mathematician, physicist, and natural philosopher.

(PRF) The pulse repetition frequency is the number of pulses per second. The usual range for echocardiographs is between 200 and 5000 pulses per second. The PRF varies with the type of mode in operation.

See also Q-value.