Ultrasound biomicroscopy utilizes high frequency (10 - 50 MHz) diagnostic ultrasound to examine living tissue at a microscopic level and allows to image the skin with extremely high resolution to a depth of 2-3 centimeters. Ultrasound biomicroscopy images provide detailed anatomical information that can lead to better and more accurate treatments and avoid a biopsy.
Ultrasound biomicroscopy improves also the spatial resolution of US images of the anterior segment of the eye. US biomicroscopy of the eye operates in the 50 MHz range with a possible axial resolution on the order of 30 μm. In this frequency range, tissue penetration of only approximately 5 mm is attainable. Both continuous wave Doppler and high-frequency pulsed Doppler can be used.

See also Ultrasound Imaging Procedures, A-Scan, B-Scan and C-Scan.

A liver sonography is a diagnostic tool to image the liver and adjoining upper abdominal organs such as the gallbladder, spleen, and pancreas. Deeper structures such as liver and pancreas are imaged at a lower frequency 1-6 MHz with lower axial and lateral resolution but greater penetration. The diagnostic capabilities in this area can be limited by gas in the bowel scattering the sound waves.
The application of microbubbles may be useful for detection of liver lesions and for lesion characterization. Some microbubbles have a liver-specific post vascular phase where they appear to be taken up by the reticuloendothelial system (RES). Dynamic contrast enhanced scans in a similar way as with CT or MRI can be used to studying the arterial, venous and tissue phase.
After a bolus injection, early vascular enhancement is seen at around 30sec in arterialized lesions (e.g., hepatocellular carcinomas (HCC), focal nodular hyperplasia (FNH)). Later enhancement is typical of hemangiomas with gradually filling towards the center. In the late phase at around 90sec, HCCs appear as defects against the liver background. Most metastases are relatively hypovascular and so do not show much enhancement and are seen as signal voids in the different phases.
Either with an intermittent imaging technique or by continuous scanning in a nondestructive, low power mode, characteristic time patterns can be used to differentiate lesions.

See also Medical Imaging, B-Mode, High Intensity Focused Ultrasound, Ultrasound Safety and Contrast Medium.

A phantom is used to control the imaging performance of ultrasound transducers. The spatial resolution, dead zone, linear fidelity, depth of penetration and image uniformity is important for the image quality. For the axial and lateral resolution, the standard definition is the resolution of objects parallel and perpendicular to the path of the sound beam. Ultrasound pictures created by scans of specially designed ultrasound phantoms can quantify the imaging quality and transducer performance.
Phantoms contain one or more materials that simulate a tissue in its interaction with ultrasound. Several phantoms are available specifically for quality control. Transducer characterization consists of a standard pulse echo analysis and insertion loss measurement for each probe. The quality variation from the baseline should be tracked over a period.